First molecular method for discriminating between Candida africana, Candida albicans, and Candida dubliniensis by using hwp1 gene

The authors report here a polymerase chain reaction-based assay using a single primer pair (CR-f/CR-r), allowing discrimination between Canclicla africana and Candida albicans by using the hwp1 gene. The method also identifies Candida dubliniensis because it produces 3 different DNA fragments: approximately 700 bp for C. africana, 941 bp for C. albicans, and 569 for C. dubliniensis. (C) 2008 Elsevier Inc. All rights reserved.