期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 60, 期 1, 页码 125-128出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2007.08.003
关键词
metallo-beta-lactamase; P. aeruginosa; bum patient; etest; PCR
The antimicrobial activities of 6 common antimicrobial agents including carbapenems were tested against 100 clinical Pseudomonas aeruginosa isolates using a disk diffusion method. All imipenem (IPM)-resistant isolates were screened for metallo-beta-lactamase (MBL) production by Etest assay and were subsequently subjected to polymerase chain reaction (PCR) analysis with the bla(IMP) and bla(VIM) genes. Of 41 IPM-nonsusceptible isolates detected, 8 (19.51%) appeared to produce MBL, as determined by Etest. Using PCR assay, these isolates were positive for bla(VIM) genes, whereas none were positive for bla(IMP) genes. (c) 2008 Elsevier Inc. All rights reserved.
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