期刊
DIABETOLOGIA
卷 57, 期 10, 页码 2165-2172出版社
SPRINGER
DOI: 10.1007/s00125-014-3316-7
关键词
DNA methylation; Inflammation; Insulin resistance; VitaminD deficiency
资金
- State Key Program of National Natural Science of China [81130049]
- National `Twelfth Five-Year' Plan for Science Technology [2012BAI02B02]
- Program for New Century Excellent Talents in University of Ministry of Education of China [NCET-10-0148]
Aims/hypothesis We aimed to investigate the impact of maternal vitamin D deficiency during pregnancy on insulin resistance in male offspring and examine its mechanism. Methods Pregnant Sprague-Dawley rats were maintained on a vitamin-D-free diet with ultraviolet-free light during pregnancy (early-VDD group). Insulin resistance in the male offspring was assessed by HOMA-IR, OGTT and euglycaemic clamp. NEFA, oxidative stress and inflammation levels were estimated as risk factors for insulin resistance. DNA methylation was examined by bisulfate sequencing PCR analysis. Luciferase reporter assay was performed to validate the effect of DNA methylation. Results The offspring in the early-VDD group had significantly higher fasting insulin and HOMA-IR levels, markedly reduced glucose tolerance and significantly lower tissue sensitivity to exogenous insulin at 16 weeks (all p < 0.05) compared with control offspring. Significantly higher serum and liver IL-1 beta, IL-6, IL-8 and TNF-alpha concentrations were observed in the offspring of the early-VDD group at 0, 3, 8 and 16 weeks. Expression of hepatic I kappa b alpha (also known as Nfkbia) mRNA and nuclear factor kappa B inhibitor alpha (I kappa B alpha) protein was persistently lower in the early-VDD offspring at all time points, and their hepatic I kappa b alpha methylation levels at the cytosine phosphate guanine site +331 were significantly higher at 0 and 16 weeks (all p < 0.01). Methylation at I kappa b alpha first exon +331 markedly decreased the luciferase activity (p < 0.05). Conclusions/interpretation Maternal vitamin D deficiency during pregnancy results in insulin resistance in the offspring, which is associated with persistently increased inflammation. Persistently decreased I kappa b alpha expression, potentially caused by changes in I kappa b alpha methylation, plays an important role in persistent inflammation.
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