期刊
DIABETOLOGIA
卷 56, 期 7, 页码 1547-1556出版社
SPRINGER
DOI: 10.1007/s00125-013-2901-5
关键词
Diabetes; Glucose stimulation; IA-2 beta; MicroRNA; miR-153; Neurodegeneration
资金
- National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, USA
- Marie Curie Intra-European Fellowship (EIF) [041333]
- Flemish community
- University of Leuven, Belgium
- European Research Commission (ERC) grant (EU Commission)
We analysed the genomic organisation of miR-153, a microRNA embedded in genes that encode two of the major type 1 diabetes autoantigens, islet-associated protein (IA)-2 and IA-2 beta. We also identified miR-153 target genes that correlated with IA-2 beta localisation and function. A bioinformatics approach was used to identify miR-153's genomic organisation. To analyse the co-regulation of miR-153 and IA-2 beta, quantitative PCR analysis of miR-153 and Ia-2 beta (also known as Ptprn2) was performed after a glucose stimulation assay in MIN6B cells and isolated murine pancreatic islets, and also in wild-type Ia-2 (also known as Ptprn), Ia-2 beta single knockout and Ia-2/Ia-2 beta double knockout mouse brain and pancreatic islets. Bioinformatics identification of miR-153 target genes and validation via luciferase reporter assays, western blotting and quantitative PCR were also carried out. Two copies of miR-153, miR-153-1 and miR-153-2, are localised in intron 19 of Ia-2 and Ia-2 beta, respectively. In rodents, only miR-153-2 is conserved. We demonstrated that expression of miR-153-2 and Ia-2 beta in rodents is partially co-regulated as demonstrated by a strong reduction of miR-153 expression levels in Ia-2 beta knockout and Ia-2/Ia-2 beta double knockout mice. miR-153 levels were unaffected in Ia-2 knockout mice. In addition, glucose stimulation, which increases Ia-2 and Ia-2 beta expression, also significantly increased expression of miR-153. Several predicted targets of miR-153 were reduced after glucose stimulation in vitro, correlating with the increase in miR-153 levels. This study suggests the involvement of miR-153, IA-2 beta and miR-153 target genes in a regulatory network, which is potentially relevant to insulin and neurotransmitter release.
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