期刊
DIABETOLOGIA
卷 55, 期 8, 页码 2226-2237出版社
SPRINGER
DOI: 10.1007/s00125-012-2581-6
关键词
Apoptosis; c-Myc; Catalytic antioxidant; Cytosolic calcium concentration; Haem oxygenase 1; Insulin secretion; Low glucose concentration; Metallothionein; Mitochondria; MnTBAP; Oxidative stress; Pancreatic beta cell; roGFP
资金
- Fonds de la Recherche Scientifique Medicale (Belgium) [3.4516.09]
- Fonds Special de Recherche from Universite catholique de Louvain
- Interuniversity Poles of Attraction Program-Belgian Science Policy [P6/40]
- Societe Francophone du Diabete
- FRIA
Pancreatic beta cells chronically exposed to low glucose concentrations show signs of oxidative stress, loss of glucose-stimulated insulin secretion (GSIS) and increased apoptosis. Our aim was to confirm the role of mitochondrial oxidative stress in rat islet cell apoptosis under these culture conditions and to evaluate whether its reduction similarly improves survival and GSIS. Apoptosis, oxidative stress-response gene mRNA expression and glucose-induced stimulation of mitochondrial metabolism, intracellular Ca2+ concentration and insulin secretion were measured in male Wistar rat islets cultured for 1 week in RPMI medium containing 5-10 mmol/l glucose with or without manganese(III)tetrakis(4-benzoic acid)porphyrin (MnTBAP) or N-acetyl-l-cysteine (NAC). Oxidative stress was measured in islet cell clusters cultured under similar conditions using cytosolic and mitochondrial redox-sensitive green fluorescent protein (roGFP1/mt-roGFP1). Prolonged culture in 5 vs 10 mmol/l glucose increased mt-roGFP1 (but not roGFP1) oxidation followed by beta cell apoptosis and loss of GSIS resulting from reduced insulin content, mitochondrial metabolism, Ca2+ influx and Ca2+-induced secretion. Tolbutamide-induced, but not high K+-induced, Ca2+ influx was also suppressed. Under these conditions, MnTBAP, but not NAC, triggered parallel similar to 50-70% reductions in mt-roGFP1 oxidation and beta cell apoptosis, but failed to protect against the loss of GSIS despite significant improvement in glucose-induced and tolbutamide-induced Ca2+ influx. Mitochondrial oxidative stress contributes differently to rat pancreatic islet cell apoptosis and insulin secretory defects during culture in a low glucose concentration. Thus, targeting beta cell survival may not be sufficient to restore insulin secretion when beta cells suffer from prolonged mitochondrial oxidative stress, e.g. in the context of reduced glucose metabolism.
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