期刊
DIABETES
卷 62, 期 7, 页码 2368-2379出版社
AMER DIABETES ASSOC
DOI: 10.2337/db12-1220
关键词
-
资金
- Spanish Ministry of Science and Innovation [SAF 2010-15239, BFU 2008-04043, SAF 2012-36186]
- Ramon y Cajal tenure track program of the Spanish Ministry of Science and Innovation [RYC 2009-04669]
- Fondo de Investigacion Sanitaria (FIS) Miguel Servet [CP10/00438]
- European Regional Development Fund (ERDF)
- Spanish Ministry of Economy and Competition
- Pro-CNIC Foundation
- Spanish Ministry
Adipose-derived stem cells (ASCs) are promising candidates for autologous cell-based regeneration therapies by virtue of their multilineage differentiation potential and immunogenicity; however, relatively little is known about their role in adipose tissue physiology and dysfunction. Here we evaluated whether ASCs isolated from nonobese and obese tissue differed in their metabolic characteristics and differentiation potential. During differentiation to mature adipocytes, mouse and human ASCs derived from nonobese tissues both increased their insulin sensitivity and inhibition of lipolysis, whereas obese-derived ASCs were insulin-resistant, showing impaired insulin-stimulated glucose uptake and resistance to the antilipolytic effect of insulin. Furthermore, obese-derived ABCs showed enhanced release of proinflammatory cytokines and impaired production of adiponectin. Interestingly, the delivery of cytosol from control ABCs into obese-derived ASCs using a lipid-based, protein-capture methodology restored insulin sensitivity on glucose and lipid metabolism and reversed the proinflammatory cytokine profile, in part due to the restoration of Lin28 protein levels. In conclusion, glucose and lipid metabolism as well as maturation of ABCs is truncated in an obese environment. The reversal of the altered pathways in obese cells by delivery of normal subcellular fractions offers a potential new tool for cell therapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据