期刊
DIABETES
卷 59, 期 4, 页码 947-957出版社
AMER DIABETES ASSOC
DOI: 10.2337/db09-0498
关键词
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资金
- National Institutes of Health [5RO1-DK-070011, AI-50864]
- American Diabetes Association [1-09-RA-413, 1-05-RA-105]
- Juvenile Diabetes Research Foundation (JDRF) [1-2005-257, 3-2008-32, 4-2008-811, JDRF-4-2004-361]
- Diabetes Research Institute Foundation, Hollywood, FL
- Diabetes Research Institute Cell Transplant Center, Islet Cell Resource Center (National Center for Research Resources) [3U42RR016603-06S1, M01RR16587]
- University of Miami
- Histology Core
- Sylvester Cancer Center
- NATIONAL CENTER FOR RESEARCH RESOURCES [M01RR016587, U42RR016603] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U19AI050864] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [DP3DK085696, R01DK070011] Funding Source: NIH RePORTER
OBJECTIVE-To investigate if recurrent autoimmunity explained hyperglycemia and C-peptide loss in three immunosuppressed simultaneous pancreas-kidney (SPK) transplant recipients. RESEARCH DESIGN AND METHODS-We monitored autoantibodies and autoreactive T-cells (using tetramers) and performed biopsy. The function of autoreactive T-cells was studied with in vitro and in vivo assays. RESULTS-Autoantibodies were present pretransplant and persisted on follow-up in one patient. They appeared years after transplantation but before the development of hyperglycemia in the remaining patients. Pancreas transplant biopsies were taken within similar to 1 year from hyperglycemia recurrence and revealed loss and insulitis. We studied autoreactive T-cells from the time of biopsy and repeatedly demonstrated their presence on further follow-up, together with autoantibodies. Treatment with T-cell-directed therapies (thymoglobulin and daclizumab, all patients), alone or with the addition of B-cell-directed therapy (rituximab, two patients), nonspecifically depleted T-cells and was associated with C-peptide secretion for >1 year. Autoreactive T-cells with the same autoantigen specificity and conserved T-cell receptor later reappeared with further C-peptide loss over the next 2 years. Purified autoreactive CD4 T-cells from two patients were cotransplanted with HLA-mismatched human islets into immunodeficient mice. Grafts showed beta-cell loss in mice receiving autoreactive T-cells but not control T-cells. CONCLUSIONS-We demonstrate the cardinal features of recurrent autoimmunity in three such patients, including the reappearance of CD4 T-cells capable of mediating B-cell destruction. Markers of autoimmunity can help diagnose this underappreciated cause of graft loss. Immune monitoring during therapy showed that autoimmunity was not resolved by the immunosuppressive agents used. Diabetes 59:947-957, 2010
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