期刊
DIABETES
卷 59, 期 1, 页码 43-50出版社
AMER DIABETES ASSOC
DOI: 10.2337/db09-1128
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资金
- The Harold L. Dorris Neurological Research Institute
- The Skaggs Institute for Chemical Biology
- The Ellison Medical Foundation, Pfizer [SFP1729]
- National Institutes of Health [R01NS043501, R01NS060799, DP1-OD-04117, AG 9900, AG028040]
- Health Research Formula Funds
- Commonwealth of Pennsylvania
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS060799, R01NS043501] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG009900, R01AG028040, R37AG009900] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [DP1OD004117] Funding Source: NIH RePORTER
OBJECTIVE-Temperature and nutrient homeostasis are two interdependent components of energy balance regulated by distinct sets of hypothalamic neurons. The objective is to examine the role of the metabolic signal insulin in the control of core body temperature (CBT). RESEARCH DESIGN AND METHODS-The effect of preoptic area administration of insulin on CBT in mice was measured by radiotelemetry and respiratory exchange ratio. In vivo 2-[F-18]fluoro-2-deoxyglucose uptake into brown adipose tissue (BAT) was measured in rats after insulin treatment by positron emission tomography combined with X-ray computed tomography imaging. Insulin receptor-positive neurons were identified by retrograde tracing from the raphe pallidus. Insulin was locally applied on hypothalamic slices to determine the direct effects of insulin on intrinsically warm-sensitive neurons by inducing hyperpolarization and reducing firing rates. RESULTS-Injection of insulin into the preoptic area of the hypothalamus induced a specific and dose-dependent elevation of CBT mediated by stimulation of BAT thermogenesis as shown by imaging and respiratory ratio measurements. Retrograde tracing indicates that insulin receptor-expressing warm-sensitive neurons activate BAT through projection via the raphe pallidus. Insulin applied on hypothalamic slices acted directly on intrinsically warm-sensitive neurons by inducing hyperpolarization and reducing firing rates. The hyperthermic effects of insulin were blocked by pretreatment with antibodies to insulin or with a phosphatidylinositol 3-kinase inhibitor. CONCLUSIONS-Our findings demonstrate that, insulin can directly modulate hypothalamic neurons that, regulate thermogenesis and CBT and indicate that insulin plays an important role in coupling metabolism and thermoregulation at the level of anterior hypothalamus. Diabetes 59:43-50, 2010
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