4.7 Article

B-cells promote intra-islet CD8+ cytotoxic T-cell survival to enhance type 1 diabetes

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DIABETES
卷 57, 期 4, 页码 909-917

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AMER DIABETES ASSOC
DOI: 10.2337/db07-1256

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  1. Wellcome Trust Funding Source: Medline

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OBJECTIVE - To determine the role of B-cells in promoting CD8+ T-cell-mediated P cell destruction in chronically inflamed islets. RESEARCH DESIGN AND METHODS - RIP-TNF alpha-NOD mice were crossed to B-cell-deficient NOD mice, and diabetes development was monitored. We used in vitro antigen presentation assays and in vivo administration of bromodeoxyuridine coupled to flow cytometry assays to assess intra-islet T-cell activation in the absence or presence of B-cells. CD(4+)Foxp(3+) activity in the absence or presence of B-cells was tested using in vivo depletion techniques. Cytokine production and apoptosis assays determined the capacity of CD8+ T-cells transform to cytotoxic T-lymphocytes (CTLs) and survive within inflamed islets in the absence or presence of B-cells. RESULTS - B-cell deficiency significantly delayed diabetes development in chronically inflamed islets. Reintroduction of B-ells incapable of secreting immunoglobulin restored diabetes development. Both CD4+ and CD8+ T-cell activation was unimpaired by B-cell deficiency, and delayed disease was not due to CD(4+)Foxp(3+) T-cell suppression of T-cell responses. Instead, at the CTL transition stage, B-cell deficiency resulted in apoptosis of intra-islet CTLs. CONCLUSIONS - In inflamed islets, B-cells are central for the efficient intra-islet survival of CTLs, thereby promoting type 1 diabetes development.

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