期刊
DEVELOPMENTAL NEUROSCIENCE
卷 33, 期 3-4, 页码 210-221出版社
KARGER
DOI: 10.1159/000327246
关键词
Hypoxic-ischemic encephalopathy; Neuroprotection; Perinatal asphyxia
资金
- NIH through the National Institute of Child Health and Human Development [HD52820, HD02274]
- ARRA (American Recovery and Reinvestment Act)
- University of Washington Institute of Translational Health Sciences National Center for Research Resources [RR025014, RR025015, RR025016]
- Washington Regional Primate Research Center [RR000166]
Perinatal asphyxia is a leading cause of brain injury in neonates, occurring in 2-4 per 1,000 live births, and there are limited treatment options. Because of their similarity to humans, nonhuman primates are ideal for performing preclinical tests of safety and efficacy for neurotherapeutic interventions. We previously developed a primate model of acute perinatal asphyxia using 12-15 min of umbilical cord occlusion. Continuing this research, we have increased cord occlusion time from 15 to 18 min and extended neurodevelopmental follow-up to 9 months. The purpose of this report is to evaluate the increase in morbidity associated with 18 min of asphyxia by comparing indices obtained from colony controls, nonasphyxiated controls and asphyxiated animals. Pigtail macaques were delivered by hysterotomy after 0, 15 or 18 min of cord occlusion, then resuscitated. Over the ensuing 9 months, for each biochemical and physiologic parameters, behavioral and developmental evaluations, and structural and spectroscopic MRI were recorded. At birth, all asphyxiated animals required resuscitation with positive pressure ventilation and exhibited biochemical and clinical characteristics diagnostic of hypoxic-ischemic encephalopathy, including metabolic acidosis and attenuated brain activity. Compared with controls, asphyxiated animals developed long-term physical and cognitive deficits. This preliminary report characterizes the acute and chronic consequences of perinatal asphyxia in a nonhuman primate model, and describes diagnostic imaging tools for quantifying correlates of neonatal brain injury as well as neurodevelopmental tests for evaluating early motor and cognitive outcomes. Copyright (C) 2011 S. Karger AG, Basel
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