期刊
DEVELOPMENTAL DYNAMICS
卷 242, 期 5, 页码 401-413出版社
WILEY
DOI: 10.1002/dvdy.23925
关键词
cornea; lens; epidermis; specification; commitment
资金
- PNR Vision [V0602]
- Vision Research fellowship
- AFM fellowship
- FRM fellowship
Background: The corneal epithelium (CE) overlays a stroma, which is derived from neural crest cells, and appears to be committed during chick development, but appears still labile in adult rabbit. Its specification was hitherto regarded as resolved and dependent upon the lens, although without experimental support. Here, we challenged CE fate by changing its environment at different stages. Results: Recombination with a dermis showed that CE commitment is linked to stroma formation, which results in Pax6 stabilization in both species. Surgical ablation shows that CE specification has already taken place when the lens placode invaginates, while removal of the early lens placode led to lens renewal. To block lens formation, bone morphogenetic protein (BMP) signaling, one of its last inducing factors, was inhibited by over-expression of Gremlin in the ocular ectoderm. This resulted in lens-less embryos which formed a corneal epithelium if they survived 2 weeks. Conclusion: The corneal epithelium and lens share a common pool of precursors. The adoption of the CE fate might be dependent on the loss of a lens placode favoring environment. The corneal fate is definitively stabilized by the migration of Gremlin-expressing neural crest cells in the lens peripheral ectoderm. Developmental Dynamics 242:401-413, 2013. (C) 2013 Wiley Periodicals, Inc.
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