期刊
DEVELOPMENTAL DYNAMICS
卷 241, 期 9, 页码 1465-1472出版社
WILEY-BLACKWELL
DOI: 10.1002/dvdy.23828
关键词
microRNA; Dicer; tooth development; Wnt1Cre; ShhCre; epithelial-mesenchymal interaction
资金
- Nihon University
- JSPS
- Grants-in-Aid for Scientific Research [23792215] Funding Source: KAKEN
- Medical Research Council [G0901599, MR/J006742/1] Funding Source: researchfish
- MRC [G0901599] Funding Source: UKRI
Background: Tooth development is known to be mediated by the cross-talk between signaling pathways, including Shh, Fgf, Bmp, and Wnt. MicroRNAs (miRNAs) are 19- to 25-nt noncoding small single-stranded RNAs that negatively regulate gene expression by binding target mRNAs, which is believed to be important for the fine-tuning signaling pathways in development. To investigate the role of miRNAs in tooth development, we examined mice with either mesenchymal (Wnt1Cre/Dicerfl/fl) or epithelial (ShhCre/Dicerfl/fl) conditional deletion of Dicer, which is essential for miRNA processing. Results: By using a CD1 genetic background for Wnt1Cre/Dicerfl/fl, we were able to examine tooth development, because the mutants retained mandible and maxilla primordia. Wnt1Cre/Dicerfl/fl mice showed an arrest or absence of teeth development, which varied in frequency between incisors and molars. Extra incisor tooth formation was found in ShhCre/Dicerfl/fl mice, whereas molars showed no significant anomalies. Microarray and in situ hybridization analysis identified several miRNAs that showed differential expression between incisors and molars. Conclusion: In tooth development, miRNAs thus play different roles in epithelium and mesenchyme, and in incisors and molars. Developmental Dynamics 241:1465-1472, 2012. (c) 2012 Wiley Periodicals, Inc.
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