期刊
DEVELOPMENTAL DYNAMICS
卷 240, 期 4, 页码 874-889出版社
WILEY-BLACKWELL
DOI: 10.1002/dvdy.22586
关键词
zebrafish; gr mRNA; morpholino (MO); development
资金
- Progetti di Ricerca di Ateneo [CPDA078853]
- University of Padova, Padova, Italy
In zebrafish, ovulated oocytes contain both maternal cortisol and the mRNA for the glucocorticoid receptor (gr), which is spread as granular structures throughout the ooplasm. At 0.2 hpf, this transcript is relocated in the blastodisc area and partitioned among blastomeres. At 6-8 hpf, it is replaced by zygotic transcript. We used morpholinos to block translation of both maternal and zygotic gr transcripts, and a missplicing morpholino to block post-transcriptionally the zygotic transcript alone. Only knockdown of translation produced an increase of apoptosis and subsequent craniofacial and caudal deformities with severe malformations of neural, vascular, and visceral organs in embryos and 5-dpf larvae. Such defects were rescued with trout gr2 mRNA. Microarray analysis revealed that 114 and 37 highly expressed transcripts were up-and down-regulated, respectively, by maternal Gr protein deficiency in 5-hpf embryos. These results indicate that the maternal gr transcript and protein participate in the maternal programming of zebrafish development. Developmental Dynamics 240:874-889, 2011. (C) 2011 Wiley-Liss, Inc.
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