期刊
DEVELOPMENTAL DYNAMICS
卷 239, 期 6, 页码 1748-1754出版社
WILEY
DOI: 10.1002/dvdy.22317
关键词
hematopoiesis; hematopoietic stem cells; functional overexpression; Runx1; Runtb2; Lmo2
资金
- Spanish Ministry of Science and Innovation [SAF2003-04317, RD06/0010/0008, BFU2009-08331/BMC]
Hematopoiesis is the dynamic process whereby blood cells are continuously produced in an organism. Blood cell production is sustained by a population of self-renewing multipotent hematopoietic stem cells (HSCs) throughout the life of an organism. Cells with definitive HSC properties appear in the mid-gestation embryo as dense clusters of cells budding from the floor of the aorta, and that of the vitelline and umbilical arteries in the aorta-gonads-mesonephros region. Attempts to genetically modify the aortic floor from which these HSCs arise have been unsuccessful in the mouse, since the regulation of gene expression in the hemogenic endothelium is largely unknown. Here we report the implementation of gene transfer by electroporation into dorsal aortic endothelial cells in the chick embryo. This approach provides a quick and reproducible method of generating gain/loss-of-function models to investigate the function of genes involved in HSC birth. Developmental Dynamics 239:1748-1754, 2010. (C) 2010 Wiley-Liss, Inc.
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