期刊
DEVELOPMENTAL DYNAMICS
卷 239, 期 2, 页码 559-573出版社
WILEY
DOI: 10.1002/dvdy.22180
关键词
pHMA; cell engulfment; Drosophila
资金
- National Institutes of Health [1R21 HD049554]
- American Cancer Society [PF-07-118-01-DDC]
- NIH National Technology Center for Networks and Pathways [5U54 RR022241]
Engulfment of apoptotic cells by phagocytosis ensures the removal of unwanted and defective cells. We developed a genetically encoded marker for cell engulfment, pHMA, which consists of the pH-Sensitive derivative of GFP, pHluorin, fused to the actin-binding domain of Moesin. In healthy cells of Drosophila embryos and cultured cells, pHMA resides at the cell cortex. In dying cells, pHMA loses its cortical localization and reports a modest decrease in pH. In embryos, the dying cells lose their apical contacts, then move basally and are ultimately engulfed by neighboring cells or macrophages. The cell corpse material is strongly acidified soon after engulfment and persists in the phagocytic cell for several hours. Changes in the pHMA signal correlate well with increases or decreases in apoptosis. These data show that pHMA is a useful reporter for cell engulfment and can be used in screening for mutations that affect cell engulfment. Developmental Dynamics 239:559-573, 2010. (C) 2009 Wiley-Liss, Inc.
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