期刊
DEVELOPMENTAL DYNAMICS
卷 238, 期 1, 页码 162-170出版社
WILEY
DOI: 10.1002/dvdy.21811
关键词
chicken embryo; heart development; coronary development; Notch1ICD; pro-epicardial organ; epicardium-derived cells
资金
- NIH [HL65314, HL075436, ES013607]
- AHA [TRN104519]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL075436, R01HL065314] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R21ES013507] Funding Source: NIH RePORTER
Notch1 is an important regulator of intercellular interactions in cardiovascular development. We show that the nuclear-localized, cleaved and active form of Notch1, the Notch1 intracellular domain (N1ICD), appeared in mesothelial cells of the pro-epicardium during epicardial formation at looped heart stages. N1ICD was also present in mesothelial cells and mesenchymal cells specifically within the epicardium at sulcus regions. N1ICD-positive endothelial cells were detected within the nascent vessel plexus at the atrioventricular junction and within the compact myocardium (Hamburger and Hamilton stage [HH] 25-HH30). The endothelial cells expressing N1ICD were surrounded by N1ICD-positive smooth muscle cells after coronary orifice formation (HH32-HH35), while N1ICD expression was absent in the mesenchymal and mesothelial cells surrounding mature coronary vessels. We propose that differential activation of the hypoxia/HIF1-VEGF-Notch pathway may play a role in epicardial cell interactions that promote epicardial epithelial/mesenchymal transition and coronary progenitor cell differentiation during epicardial development and coronary vasculogenesis in particularly hypoxic sulcus regions. Developmental Dynamics 238:162-170, 2009. (C) 2008 Wiley-Liss, Inc.
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