期刊
DEVELOPMENTAL DYNAMICS
卷 238, 期 8, 页码 2025-2033出版社
WILEY-BLACKWELL
DOI: 10.1002/dvdy.22029
关键词
cricket; Fragile X mental retardation; Dopamine receptor; RNA interference; wing posture; calling song; circadian locomotor rhythm
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
In order to explore a possibility that the cricket Gryllus bimaculatus would be a useful model to unveil molecular mechanisms of human diseases, we performed loss-of-function analyses of Gryllus genes homologous to human genes that are responsible for human disorders, fragile X mental retardation 1 (fmr1) and Dopamine receptor (DopR). We cloned cDNAs of their Gryllus homologues, Gb'fmr1, Gb'DopRI, and Gb'DopRII, and analyzed their functions with use of nymphal RNA interference (RNAi). For Gb'fmr1, three major phenotypes were observed: (1) abnormal wing postures, (2) abnormal calling song, and (3) loss of the circadian locomotor rhythm, while for Gb'DopRI, defects of wing posture and morphology were found. These results indicate that the cricket has the potential to become a novel model system to explore human neuronal pathogenic mechanisms and to screen therapeutic drugs by RNAi. Developmental Dynamics 238. 2025-2033, 2009. (C) 2009 Wiley-Liss, Inc.
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