期刊
DEVELOPMENTAL DYNAMICS
卷 238, 期 1, 页码 138-149出版社
WILEY
DOI: 10.1002/dvdy.21833
关键词
muscle stem cell; satellite cell; Pax7; myogenic differentiation; skeletal muscle
资金
- NIH [RO1 DE 015404]
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [R01DE015404] Funding Source: NIH RePORTER
Studies using mouse models have established a critical role for resident satellite stem cells in skeletal muscle development and regeneration, but little is known about this paradigm in human muscle. Here, using human muscle stem cells, we address their lineage progression, differentiation, migration, and self-renewal. Isolated human satellite cells expressed alpha 7-integrin and other definitive muscle markers, were highly motile on laminin substrates and could undergo efficient myotube differentiation and myofibrillogenesis. However, only a subpopulation of the myoblasts expressed Pax7 and displayed a variable lineage progression as measured by desmin and MyoD expression. Analysis identified a differentiation-resistant progenitor cell population that was Pax7(+)/desmin(-) and capable of self-renewal. This study extends our understanding of the role of Pax7 in regulating human satellite stem cell differentiation and self-renewal. Developmental Dynamics 238:138-149, 2009. (C) 2008 Wiley-Liss, Inc.
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