期刊
DEVELOPMENTAL DYNAMICS
卷 237, 期 10, 页码 2874-2888出版社
WILEY
DOI: 10.1002/dvdy.21709
关键词
apical membrane; Crumbs; live imaging; mechanics; Moesin; Ribbon; tube morphogenesis
资金
- NIH NIDCR [R01 DE12873, R01 DE13899]
- NIH NIBIB [P41 EB01976]
- Whitaker Foundation Graduate Fellowship
Ribbon is a nuclear Broad Tramtrack Bric-a-brac (BTB) -domain protein required for morphogenesis of the salivary gland and trachea. We recently showed that ribbon mutants exhibit decreased Crumbs and Rab11-coincident apical vesicles and increased apical Moesin activity and microvillar structure during tube elongation. To learn how these molecular and morphological changes affect the dynamics of tubulogenesis, we optimized an advanced two-photon microscope to enable high-resolution live imaging of the salivary gland and trachea. Live imaging revealed that ribbon mutant tissues exhibit slowed and incomplete lumenal morphogenesis, consistent with previously described apical defects. Because Moesin activity correlates with cortical stiffness, we hypothesize that ribbon mutants suffer from increased apical stiffness during morphogenesis. We develop this hypothesis through mechanical analysis, using the advantages of live imaging to construct computational elastic and analytical viscoelastic models of tube elongation, which suggest that ribbon mutant tubes exhibit three- to fivefold increased apical stiffness and twofold increased effective apical viscosity. Developmental Dynamics 237:2874-2888, 2008. (C) 2008 Wiley-Liss, Inc.
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