期刊
DEVELOPMENTAL DYNAMICS
卷 237, 期 5, 页码 1348-1358出版社
WILEY-BLACKWELL
DOI: 10.1002/dvdy.21533
关键词
spindle; anastral; sea urchin; centrosome; kinesin
资金
- Howard Hughes Medical Institute Funding Source: Medline
- NIBIB NIH HHS [R01 EB002583, R01 EB002583-13] Funding Source: Medline
- NIGMS NIH HHS [GM060925-2, R15 GM060925] Funding Source: Medline
The mitotic apparatus of the early sea urchin embryo is the archetype example of a centrosome-dominated, large aster spindle organized by means of the centriole of the fertilizing sperm. In this study, we tested the hypothesis that artificially activated sea urchin eggs possess the capacity to assemble the anastral, bipolar spindles present in many acentrosomal systems. Control fertilized Lytechinus pictus embryos and ammonia-activated eggs were immunolabeled for tubulin, centrosomal material, the spindle pole structuring protein NuMA and the mitotic kinesins MKLP1/Kinesin-6, Eg5/Kinesin-5, and KinI/Kinesin-13. Confocal imaging showed that a subset of ammonia-activated eggs contained bipolar mini-spindles that were anastral; displayed metaphase and anaphase-like stages; labeled for centrosomal material, NuMA, and the three mitotic kinesins; and were observed in living eggs using polarization optics. These results suggest that spindle structural and motor proteins have the ability to organize bipolar, anastral spindles in sea urchin eggs activated in the absence of the paternal centriole.
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