期刊
DEVELOPMENTAL DYNAMICS
卷 237, 期 4, 页码 1119-1132出版社
WILEY
DOI: 10.1002/dvdy.21498
关键词
Phox2b; transgene; BAC modification; neural crest; enteric nervous system; glia; fluorescent protein; CFP
资金
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [P30HD015052] Funding Source: NIH RePORTER
- NATIONAL CANCER INSTITUTE [P30CA068485] Funding Source: NIH RePORTER
- NATIONAL EYE INSTITUTE [P30EY008126] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R21DK064251, P30DK020593, P30DK058404, P60DK020593, U2CDK059637, U24DK059637] Funding Source: NIH RePORTER
- NCI NIH HHS [P30 CA068485, CA68485] Funding Source: Medline
- NEI NIH HHS [P30 EY008126, EY08126] Funding Source: Medline
- NICHD NIH HHS [HD15052, P30 HD015052] Funding Source: Medline
- NIDDK NIH HHS [R21 DK064251-02, R21 DK064251-01, DK59637, P30 DK020593, R21 DK064251, U24 DK059637, DK064251, P60 DK020593, DK58404, P30 DK058404, DK20593] Funding Source: Medline
The mammalian enteric nervous system (ENS) derives from migratory enteric neural crest- derived cells (ENCC) that express the transcription factor Phox2b. Studies of these enteric progenitors have typically relied on immunohistochemical (IHC) detection. To circumvent complicating factors of IHC, we have generated a mouse BAC transgenic line that drives a Histone2BCerulean (H2BCFP) reporter from Phox2b regulatory regions. This construct does not alter the endogenous Phox2b locus and enables studies of normal neural crest (NC) derivatives. The Phox2b-H2BCFP transgene expresses the H2BCFP reporter in patterns that recapitulate expression of endogenous Phox2b. Our studies reveal Phox2b expression in mature enteric glia at levels below that of enteric neurons. Moreover, we also observe differential expression of the transgene reporter within the leading ENCC that traverse the gut. Our findings indicate that the wavefront of migrating enteric progenitors is not homogeneous, and suggest these cells may be fate-specified before expression of mature lineage markers appears.
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