期刊
DEVELOPMENTAL CELL
卷 28, 期 4, 页码 459-473出版社
CELL PRESS
DOI: 10.1016/j.devcel.2014.01.020
关键词
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资金
- Damon Runyon Cancer Research fellowship
- EMBO
- Human Frontier Science Program
- NIH/NIGMS [R01-GM084947, R01-GM067761]
- NIH [GM043301]
- Stowers Institute for Medical Research
Stem cells possess the capacity to generate two cells of distinct fate upon division: one cell retaining stem cell identity and the other cell destined to differentiate. These cell fates are established by cell-type-specific genetic networks. To comprehensively identify components of these networks, we performed a large-scale RNAi screen in Drosophila female germline stem cells (GSCs) covering similar to 25% of the genome. The screen identified 366 genes that affect GSC maintenance, differentiation, or other processes involved in oogenesis. Comparison of GSC regulators with neural stem cell self-renewal factors identifies common and cell-type-specific self-renewal genes. Importantly, we identify the histone methyltransferase Set1 as a GSC-specific self-renewal factor. Loss of Set1 in neural stem cells does not affect cell fate decisions, suggesting a differential requirement of H3K4me3 in different stem cell lineages. Altogether, our study provides a resource that will help to further dissect the networks underlying stem cell self-renewal.
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