期刊
DEVELOPMENTAL CELL
卷 24, 期 4, 页码 384-399出版社
CELL PRESS
DOI: 10.1016/j.devcel.2013.01.013
关键词
-
资金
- Gladstone Institutes [R01 GM097194, R01 GM099844, R01 DK56598]
- National Institute of General Medical Sciences [F32GM096859]
- AHA NCRP Scientist Development Grant [0835203 N]
- AHA Predoctoral Fellowship [11PRE5140017]
Lipid droplets (LDs) store metabolic energy and membrane lipid precursors. With excess metabolic energy, cells synthesize triacylglycerol (TG) and form LDs that grow dramatically. It is unclear how TG synthesis relates to LD formation and growth. Here, we identify two LD subpopulations: smaller LDs of relatively constant size, and LDs that grow larger. The latter population contains isoenzymes for each step of TG synthesis. Glycerol-3-phosphate acyltransferase 4 (GPAT4), which catalyzes the first and rate-limiting step, relocalizes from the endoplasmic reticulum (ER) to a subset of forming LDs, where it becomes stably associated. ER-to-LD targeting of GPAT4 and other LD-localized TG synthesis isozymes is required for LD growth. Key features of GPAT4 ER-to-LD targeting and function in LD growth are conserved between Drosophila and mammalian cells. Our results explain how TG synthesis is coupled with LD growth and identify two distinct LD subpopulations based on their capacity for localized TG synthesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据