期刊
DEVELOPMENTAL CELL
卷 23, 期 2, 页码 412-424出版社
CELL PRESS
DOI: 10.1016/j.devcel.2012.06.001
关键词
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资金
- European Commission
- National Funds through Fundacao para a Ciencia e Tecnologia (FCT) [PTDC/BIA-BCM/105602/2008]
- FCT
- Instituto Gulbenkian de Ciencia
- EMBO YIP Program
- European Research Council (ERC) under the European Union [261344-CentrioleStructNumber]
- Ciencia program
- ERC
- EMBO
- Marie Curie Actions fellowships
- Fundação para a Ciência e a Tecnologia [PTDC/BIA-BCM/105602/2008] Funding Source: FCT
Cilia and flagella are involved in a variety of processes and human diseases, including ciliopathies and sterility. Their motility is often controlled by a central microtubule (MT) pair localized within the ciliary MT-based skeleton, the axoneme. We characterized the formation of the motility apparatus in detail in Drosophila spermatogenesis. We show that assembly of the central MT pair starts prior to the meiotic divisions, with nucleation of a singlet MT within the basal body of a small cilium, and that the second MT of the pair only assembles much later, upon flagella formation. BLD10/CEP135, a conserved player in centriole and flagella biogenesis, can bind and stabilize MTs and is required for the early steps of central MT pair formation. This work describes a genetically tractable system to study motile cilia formation and provides an explanation for BLD10/CEP135's role in assembling highly stable MT-based structures, such as motile axonemes and centrioles.
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