期刊
DEVELOPMENTAL CELL
卷 23, 期 1, 页码 166-180出版社
CELL PRESS
DOI: 10.1016/j.devcel.2012.04.019
关键词
-
资金
- INSERM
- Association Francaise contre les Myopathies (AFM)
- Association pour la Recherche sur le Cancer (ARC)
- Mairie de Paris Medical Research and Health Program
- Fondation pour la Recherche Medicale (FRM)
- Ecole des Neurosciences de Paris (ENP)
The compartmental organization of eukaryotic cells is maintained dynamically by vesicular trafficking. SNARE proteins play a crucial role in intracellular membrane fusion and need to be targeted to their proper donor or acceptor membrane. The molecular mechanisms that allow for the secretory vesicles carrying the v-SNARE TI-VAMP/VAMP7 to leave the cell center, load onto microtubules, and reach the periphery to mediate exocytosis are largely unknown. Here, we show that the TI-VAMP/VAMP7 partner Varp, a Rab21 guanine nucleotide exchange factor, interacts with GolginA4 and the kinesin 1 Kif5A. Activated Rab21-GTP in turn binds to MACF1, an actin and microtubule regulator, which is itself a partner of GolginA4. These components are required for directed movement of TI-VAMP/VAMP7 vesicles from the cell center to the cell periphery. The molecular mechanisms uncovered here suggest an integrated view of the transport of vesicles carrying a specific v-SNARE toward the cell surface.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据