期刊
DEVELOPMENTAL CELL
卷 19, 期 2, 页码 232-244出版社
CELL PRESS
DOI: 10.1016/j.devcel.2010.07.013
关键词
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资金
- Human Frontier Science Program
- Medical Research Council
- Cancer Research UK
- Wellcome Trust
- Lister Research Institute Prize
- Association for International Cancer Research
- BBSRC
- Volkswagenstiftung
- Ministry of Education, Science, Sports and Culture of Japan
- MRC [G0701147] Funding Source: UKRI
- Medical Research Council [G0701147] Funding Source: researchfish
Sister chromatid separation is initiated at anaphase onset by the activation of separase, which removes cohesins from chromosomes. However, it remains elusive how sister chromatid separation is completed along the entire chromosome length. Here we found that, during early anaphase in Saccharomyces cerevisiae, sister chromatids separate gradually from centromeres to telomeres, accompanied by regional chromosome stretching and subsequent recoiling. The stretching results from residual cohesion between sister chromatids, which prevents their immediate separation. This residual cohesion is at least partly dependent on cohesins that have escaped removal by separase at anaphase onset. Meanwhile, recoiling of a stretched chromosome region requires condensins and generates forces to remove residual cohesion. We provide evidence that condensins promote chromosome recoiling directly in vivo, which is distinct from their known function in resolving sister chromatids. Our work identifies residual sister chromatid cohesion during early anaphase and reveals condensins' roles in chromosome recoiling, which eliminates residual cohesion to complete sister chromatid separation.
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