期刊
DEVELOPMENTAL BIOLOGY
卷 375, 期 1, 页码 13-22出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2013.01.002
关键词
Ezrin phosphorylation; Embryo compaction; Mek/Erk; PKC; Rho GTPase
资金
- National Natural Science Foundation of China [31271547, 90919009, 31171381]
- National Basic Research Program of China [2009CB941000, 2010CB833603]
- Transgenic Program from Ministry of Agriculture of China [2009ZX08006-010B, 2009ZX08006-011B]
- 111 Project [B08011]
Phosphorylation of Ezrin T567 plays an important role in eight-cell embryo compaction. Yet, it is not clear how Ezrin phosphorylation is regulated during embryo compaction. Here, we demonstrated that inhibition of Mek/Erk or protein kinase C (PKC) signaling reduced the phosphorylation level of Ezrin T567 in eight-cell compacted embryos. Interestingly, the Rho GTPase inhibitor C3-transferase caused basolateral enrichment of atypical PKC (aPKC), as well as basolateral shift of phosphorylated Ezrin, suggesting aPKC may be a key regulator of Ezrin phosphorylation. Moreover, inhibition of PKC, but not Mek/Erk or Rho GTPases, affected the maintenance of Ezrin phosphorylation in compacted embryos. We further identified that aPKC is indeed required for Ezrin phosphorylation in eight-cell embryos. Taken together, Rho GTPases facilitate the apical distribution of aPKC and Ezrin. Subsequently, aPKC and Mek/Erk work together to promote Ezrin phosphorylation at the apical region, which in turn mediates the apical enrichment of filamentous actin, stabilizing the polarized apical region and allowing embryo compaction. Our data also suggested that aPKC might be the Ezrin kinase during eight-cell embryo compaction. (C) 2013 Elsevier Inc. All rights reserved.
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