期刊
DEVELOPMENTAL BIOLOGY
卷 361, 期 2, 页码 358-363出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2011.10.030
关键词
Pluripotency; Epiblast; Hypoblast; Fibroblast growth factor; Human ES cell derivation
资金
- Wellcome Trust
- Medical Research Council
- Biotechnology and Biological Sciences Research Council
- Medical Research Council [G0800784B, G1001028, G0600275, MC_PC_12009, G0800784, G1100526] Funding Source: researchfish
- MRC [G1001028, G0600275, G0800784] Funding Source: UKRI
Mouse embryos segregate three different lineages during preimplantation development: trophoblast, epiblast and hypoblast. These differentiation processes are associated with restricted expression of key transcription factors (Cdx2, Oct4, Nanog and Gata6). The mechanisms of segregation have been extensively studied in the mouse, but are not as well characterised in other species. In the human embryo, hypoblast differentiation has not previously been characterised. Here we demonstrate co-exclusive immunolocalisation of Nanog and Gata4 in human blastocysts, implying segregation of epiblast and hypoblast, as in rodent embryos. However, the formation of hypoblast in the human is apparently not dependent upon FGF signalling, in contrast to rodent embryos. Nonetheless, the persistence of Nanog-positive cells in embryos following treatment with FGF inhibitors is suggestive of a transient naive pluripotent population in the human blastocyst, which may be similar to rodent epiblast and ES cells but is not sustained during conventional human ES cell derivation protocols. (C) 2011 Elsevier Inc. All rights reserved.
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