期刊
DEVELOPMENTAL BIOLOGY
卷 359, 期 2, 页码 222-229出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2011.08.020
关键词
Wnt; Lrp5; Lrp6; beta-catenin; alpha-catenin; Osteoblast; Mouse; Chondrocyte
资金
- NIH [AR060456, DK065789, AR053293]
- Van Andel Research Institute
The role of Wnt signaling in osteoblastogenesis in the embryo remains to be fully established. Although beta-catenin, a multifunctional protein also mediating canonical Wnt signaling, is indispensable for embryonic osteoblast differentiation, the roles of the key Wnt co-receptors Lrp5 and Lrp6 are unclear. Indeed, global deletion of either Lrp5 or Lrp6 did not overtly affect osteoblast differentiation in the mouse embryo. Here, we generated mice lacking both receptors specifically in the embryonic mesenchyme and observed an absence of osteoblasts in the embryo. In addition, the double-deficient embryos developed supernumerary cartilage elements in the zeugopod, revealing an important role for mesenchymal Lrp5/6 signaling in limb patterning. Importantly, the phenotypes of the Lrp5/6 mutant closely resembled those of the beta-catenin-deficient embryos. These phenotypes are likely independent of any effect on the adherens junction, as deletion of alpha-catenin, another component of the complex, did not cause similar defects. Thus, Lrp5 and 6 redundantly control embryonic skeletal development, likely through beta-catenin signaling. (C) 2011 Elsevier Inc. All rights reserved.
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