期刊
DEVELOPMENTAL BIOLOGY
卷 360, 期 1, 页码 87-95出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2011.09.009
关键词
Apical-basal polarity; Crumbs; Retromer; Vps35; Drosophila
资金
- NIH [2R01 GM063891, 1R01GM087517]
- National Basic Research Program of China [2011CB943901]
- Chinese Academy of Sciences [KSCX2-YW-R-263, KSCX2-YW-R-240]
Epithelial cells are characterized by an apical-basal polarization. The transmembrane protein Crumbs (Crb) is an essential apical determinant which confers apical membrane identity. Previous studies indicated that Crb did not constantly reside on the apical membrane, but was actively recycled. However, the cellular mechanism(s) underlying this process was unclear. Here we showed that in Drosophila, retromer, which was a retrograde complex recycling certain transmembrane proteins from endosomes to trans-Golgi network (TGN), regulated Crb in epithelial cells. In the absence of retromer, Crb was mis-targeted into lysosomes and degraded, causing a disruption of the apical-basal polarity. We further showed that Crb co-localized and interacted with retromer, suggesting that retromer regulated the retrograde recycling of Crb. Our data presented here uncover the role of retromer in regulating apical-basal polarity in epithelial cells and identify retromer as a novel regulator of Crb recycling. (C) 2011 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据