期刊
DEVELOPMENTAL BIOLOGY
卷 320, 期 1, 页码 72-78出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2008.04.018
关键词
salivary gland; progenitor cells; submandibular sublingual; ascl3 demilune cell and parotid protein; duct cells; cre recombinase; mouse lineage tracing
资金
- NIDCR NIH HHS [2T32DE007202-17A1, R21 DE017386, T32 DE007202, R21 DE017386-01] Funding Source: Medline
Ascl3, also know as Sgn1, is a member of the mammalian achaete score (Mash) gene family Of transcription factors, which have been implicated in cell fate specification and differentiation. In the mouse salivary gland, expression of Ascl3 is restricted to a subset of duct cells. Salivary gland function depends on the secretory acinar cells, which are responsible for saliva formation, and duct cells, which modify the saliva and conduct it to the Oral cavity. The salivary gland ducts are also the putative site of progenitor cells in the adult gland. Using a Cre recombinase-mediated reporter system, we followed the fate of Ascl3-expressing cells after the introduction of an EGFP-Cre expression cassette into the Ascl3 locus by homologous recombination. Lineage tracing shows that these cells are progenitors of both acinar and ductal cell types in all three major salivary glands. In the differentiated progeny, expression of Ascl3 is down-regulated. These data directly demonstrate a progenitor-progeny relationship between duct cells and the acinar cell compartment, and identify a population of multipotent progenitor cells, marked by expression of Ascl3, which is capable of generating both gland cell types. We conclude that Ascl3-expressing cells contribute to the maintenance of the adult salivary glands. (C) 2008 Elsevier Inc. All rights reserved.
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