期刊
DEVELOPMENTAL BIOLOGY
卷 317, 期 1, 页码 282-295出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2008.02.037
关键词
Twistl; Tbx20; endocardial cushion development; Cadherin 11; Periostin; Mmp2; Aggrecan; Versican; cell proliferation; cell migration; siRNA; chicken
资金
- NHLBI NIH HHS [R01 HL082716, R01 HL082716-03, HL082716] Funding Source: Medline
Twist I is a bHLH transcription factor that regulates cell proliferation, migration, and differentiation in embryonic progenitor cell populations and transformed tumor cells. While much is known about Twistl's function in a variety of mesenchymal cell types, the role of Twistl in endocardial cushion cells is unknown. Twistl gain and loss of function experiments were performed in primary chicken endocardial cushion cells in order to elucidate its role in endocardial cushion development. These studies indicate that Twistl can induce endocardial cushion cell proliferation as well as promote endocardial cushion cell migration. Furthermore, Twistl is subject to BMP regulation and can induce expression of cell migration marker genes including Periostin, Cadherin 11, and Mmp2 while repressing markers of valve cell differentiation including Aggrecan. Previously, Tbx20 has been implicated in endocardial cushion cell proliferation and differentiation, and in the current study, Tbx20 also promotes cushion cell migration. Twistl can induce Tbx20 expression, while Tbx20 does not affect Twist] expression. Taken together, these data indicate a role for Twist] upstream of Tbx20 in promoting cell proliferation and migration and repressing differentiation in endocardial cushion cells during embryonic development. (C) 2008 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据