期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 88, 期 -, 页码 190-199出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2018.07.023
关键词
gamma delta T cells; Bovine; WC1; T cell receptor; Next generation sequencing; Neonatal immunity
资金
- USDA National Institute for Food and Agriculture [2017-67015-26631]
- AFRI competitive grant [2011-67015-30736]
- NIH from the NIFA USDA-NIH program Dual Purpose with Dual Benefit: Research in Biomedicine and Agriculture Using Agriculturally Important Domestic Species [R01 HD070056-01]
- NSERC Canada
- Dairy Farmers of Ontario
- BBSRC [BBS/E/D/20002174, BBS/E/D/10002071] Funding Source: UKRI
Here we evaluated neonatal transcription of alpha, beta, gamma and delta TCR and the gamma delta T cell co-receptor family WC1 in peripheral blood mononuclear cells. A previous report showed a rapid and global shift in transcription of immunoglobulin genes in neonatal calves during the first month after birth but this was not found here for the T cell genes. Transcription frequency of genes within TRAV subgroups correlated with the number of members, indicating a stochastic choice. In contrast, of the approximately 60 TRDV genes those in two of eleven TRDV1 clades and TRDVb3 were transcribed significantly more than the others while those in only one TRBV subgroup were. Transcription of genes in the TRGV5-containing cassette predominated among TRGV genes as a result of their exclusive usage by the WC1(+) gamma delta T cells with a preference for transcription of two of four TRGV genes in that cassette. Finally, we report no large differences in transcription frequencies among the 13 WC1 genes.
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