期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 44, 期 2, 页码 280-285出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2014.01.003
关键词
Neutrophils; Neutrophil extracellular traps (NETS); beta-Glucan; Aeromonas hydrophila; Carp
资金
- European Community's Seventh Framework Program (FP7) [PITN-GA-2008-21405]
The formation of neutrophil extracellular traps (NETs) has been characterised as a novel antimicrobial host defence strategy of neutrophils besides phagocytosis and degranulation, which may lead to entrapment and subsequent immobilisation and/or killing of bacterial pathogens. Here we studied the effect of the feed additive beta-glucan, namely MacroGard (R), on the formation and functionality of NETs in carp. Therefore, common carp (Cyprinus carpio) head kidney and kidney cells were isolated and treated with or without beta-glucan over time. The formation of NETs was analysed by immunofluorescence microscopy and revealed a distinct increase of NET-formation with beta-glucan. Furthermore the subsequent entrapment of Aeromonas hydrophila, an important fish pathogen, was increased after stimulating the cells with beta-glucan. However, beta-glucan did not lead to a stimulation of antimicrobial activity of neutrophils against A. hydrophila. In conclusion, the data underline the fact that the feed additive beta-glucan is able to modulate carp neutrophil functions. (C) 2014 Elsevier Ltd. All rights reserved.
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