期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 43, 期 1, 页码 76-86出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2013.10.015
关键词
Immunoglobulin; IgZ/T; Isotype exclusion; Evolution; Tuna
资金
- NIH [AI56963]
- NSF [IOS1257829]
- TAMU-CONACyT initiative [2010-006]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1257829] Funding Source: National Science Foundation
It is now appreciated that in addition to the immunoglobulin (Ig)M and D isotypes fish also make the mucosal IgT. In this study we sequenced the full length of tau as well as mu, in the commercially important Thunnus orientalis (Pacific bluefin tuna), the first molecular analysis of these two Ig isotypes in a member of the order Perciformes. Tuna IgM and IgT are each composed of four constant (CH) domains. We cloned and sequenced 48 different variable (VH) domain gene rearrangements of tuna immunoglobulins and grouped the VH gene sequences to four VH gene segment families based on 70% nucleotide identity. Three VH gene families were used by both IgM and IgT but one group was only found to be used by IgM. Most interestingly, both and tau clones appear to use the same diversity (DH) segment, unlike what has been described in other species, although they have dedicated IgT and IgM joining (JH) gene segments. We complemented this repertoire study with phylogenetic and tissue expression analysis. In addition to supporting the development of humoral vaccines in this important aquaculture species, these data suggest that the DH-JH recombination rather than the VH-DH recombination may be instructive for IgT versus IgM/D bearing lymphocyte lineages in some fish. (C) 2013 Elsevier Ltd. All rights reserved.
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