期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 40, 期 2, 页码 142-147出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2013.02.003
关键词
C-lectin; Immune priming; Chlamys farreri; Vibrio anguillarum
资金
- National Basic Research Program of China (973 Program) from the Chinese Ministry of Science and Technology [2010CB126404]
- National Science Foundation of China [30925028, 41006096]
- Shandong Provincial Natural Science Foundation [JQ201110]
The enhanced immunity against a second encounter with the particular pathogen has suggested the presence of immune priming in scallop. In the present study, the survival rate and expression patterns of five C-lectin isoforms from scallop Chlamys farreri were explored after vaccination of heat-killed Vibrio anguillarum or successively challenge with V. anguillarum and Micrococcus luteus. When scallops were challenged with live bacteria, the survival rate increased significantly only in the group firstly vaccinated with inactivated V. anguillarum and then challenged with live V. anguillarum compared with naive scallops (from 41% to 63.6%, P < 0.05), showing enhanced protective effects of inactivated bacteria with specificity. When scallops received the challenge with V. anguillarum, the mRNA expression level of five C-lectins in scallops which were immuned previously with heat-killed V. anguillarum peaked significantly higher (26.7-, 121.7-, 60.1-, 27.4-, 16.3-fold to Oh, respectively, P < 0.01) than that in non-immuned scallops (7.6-fold, P < 0.05; 6.4-, 3.9-fold, P > 0.05; 5.7-fold, P < 0.05; 11.7-fold, P < 0.01, to Oh, respectively). A significantly higher peak and 3-9 h earlier response of all C-lectins mRNA expression were observed after challenge with live V. anguillarum (26.7-, 121.7-, 60.1-, 26.4- and 16.3-fold to Oh, respectively, P < 0.01), compared with those only received first injection with heat-killed V. anguillarum (1.6-fold, P > 0.05; 8.3-fold, P < 0.05; 5.2-fold, P > 0.05; 14.5-fold, P < 0.01; 4.3-fold, P > 0.05, to 0 h, respectively). The response of mRNA expression to the secondary encounter with the same bacteria was stronger than that of successively encounter with the different class of bacteria. It was obvious that the mRNA expression of C-lectins in scallops was significantly enhanced by the successive challenge of same species of bacteria with a certain degree of specificity. All the results suggested that C-lectins might be involved in some form of immune priming, and it might provide new insights into mechanism of invertebrate immune priming. (C) 2013 Elsevier Ltd. All rights reserved.
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