期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 35, 期 1, 页码 105-114出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2010.08.013
关键词
Litopenaeus vannamei; LvTRAF6; Antimicrobial peptide genes; WSSV; Vibrio alginolyticus; Toll signal pathway
资金
- National High Technology Research and Development Program of China (863 Program) [2010AA10A401]
- National Key Technology R&D Program in the 11th Five-year Plan of China [2007BAD291301]
- Modern Agro-industry Technology Research System
- State Key Laboratory of Biocontrol [SKLBC09K04]
- Foundation from Science and Technology Bureau of Guangdong Province [2008A020100004, 2009A020102002]
Tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) is a key signaling adaptor protein not only for the TNFR superfamily but also for the Interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. To investigate TRAF6 function in invertebrate innate immune responses, Litopenaeus vannamei TRAF6 (LvTRAF6) was identified and characterized. The full-length cDNA of LvTRAF6 is 2823 bp long, with an open reading frame (ORF) encoding a putative protein of 594 amino acids, including a RING-type Zinc finger, two TRAF-type Zinc fingers, a coiled-coil region, and a meprin and TRAF homology (MATH) domain. The overall amino acid sequence identity between LvTRAF6 and other known TRAF6s is 22.2-33.3%. Dual luciferase reporter assays in Drosophila S2 cells revealed that LVTRAF6 could activate the promoters of antimicrobial peptide genes (AMPs), including Drosophila Attacin A and Drosomycin, and shrimp Penaeidins. Real-time quantitative PCR (qPCR) indicated that LvTRAF6 was constitutively expressed in various tissues of L vannamei. After Vibrio olginolyticus and white spot syndrome virus (WSSV) challenge, LvTRAF6 was clown-regulated, though with different expression patterns in the intestine compared to other tissues. After WSSV challenge, LvTRAF6 was up-regulated 2.7- and 2.3-fold over the control at 3h in gills and hepatopancreas, respectively. These results indicated that LvTRAF6 may play a crucial role in antibacterial and antiviral responses via regulation of AMP gene expression. (C) 2010 Elsevier Ltd. All rights reserved.
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