期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 35, 期 3, 页码 385-391出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2010.11.008
关键词
Defensin; RNase-A; Peptidoglycan recognition protein; Innate immunity; Swim bladder; Zebrafish
资金
- Foundation for Research Science and Technology of New Zealand
The zebrafish is increasingly being utilized to study aspects of the conserved innate intestinal immunity of vertebrates. In mammals, some antimicrobial proteins are synthesised by specialised immune cells that appear to have no equivalent in zebrafish. To delineate foci of antimicrobial protein production along the zebrafish intestine, we examined the antero-posterior expression gradients of antimicrobial genes. Quantitative PCR revealed distinct expression gradient profiles, with the mid-intestine exhibiting elevated expression of several genes such as dual oxidase and the defensin beta-like and peptidoglycan recognition protein families. This region also presented with the most numbers of leukocytes and endocytic cells, supporting a specialised immunological role. Conversely, expression of the Dr-RNase family was prominent in the anterior intestine. Expression of the zebrafish beta-defensin family was examined in adult zebrafish tissues. Strong expression of defensin beta-like 1 was detected in the swim bladder of zebrafish from the larval stage of development through to adults. (C) 2010 Elsevier Ltd. All rights reserved.
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