期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 34, 期 3, 页码 360-368出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2009.11.008
关键词
Stem cell antigen 2 (SCA2); Chicken; Antibody; Bursa of Fabricius; Cortical-medullary epithelial cells (CMEC); B cells
资金
- USDA NRICGP [200305414]
Stem cell antigen 2 (SCA2) is a Ly6 family member whose function is largely unknown. To characterize biological properties and tissue distribution of chicken SCA2, SCA2 was expressed in E. coli, purified, and a polyclonal antibody developed. Utilizing the polyclonal antibody, SCA2 is a 13 kDa cell surface protein anchored by a glycosyl-phosphatidylinositol (GPI) moiety. SCA2 is expressed in connective tissues of thymus and bursa based on immunohistochemistry, immunoprecipitation, and western blots. In bursal follicles, SCA2 is specifically expressed on the cortical-medullary epithelia[ cells (CMEC) surrounded by MHC class II presenting cells. Expression profiles of bursal cells induced by contact with SCA2-expressing cells shows down-regulation of numerous genes including CD79B, B cell linker (BLNK), spleen tyrosine kinase (SYK), and gamma 2-phospholipase C (PLCG2) that are involved in the B cell receptor (BCR) and immune response signaling pathways. These results suggest chicken SCA2 plays a role in regulating B lymphocytes. Published by Elsevier Ltd.
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