期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 33, 期 9, 页码 1011-1017出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2009.04.007
关键词
TLR8; MyD88; Atlantic salmon; Innate immunity; CpG; IFN
资金
- Research Council of Norway (RCN) [NFR 151879/150]
Toll-like receptor 8 (TLR 8) belongs to a subgroup of the TLR family that recognizes nucleic acids and that is involved in the protection against viruses. In mammals, TLR7 and 8 have been characterized as receptors for viral and synthetic single-stranded RNA. Here we describe the cloning of a TLR8 homolog in Atlantic salmon (Salmo salar) and its proximal adaptor protein MyD88. The mRNA expression of SsTLR8 was tissue-restricted and its highest level was detected in the spleen while SsMyD88 was expressed in all of the tested organs. SsTLR8 and SsMyD88 mRNAs were up-regulated in TO cells treated with recombinant IFN alpha 1 and IFN gamma. In vivo, the expression of SsTLR8 was not significantly affected following challenge with salmon alphavirus subtype 3 (SAV3). By contrast, infection with SAV3 up-regulated SsMyD88 transcripts on day 7 post-challenge and the expression remained elevated at day 28. The SsMyD88 expression in vivo paralleled type I IFN expression. In vitro stimulation of salmon head kidney leukocytes with CpG ODNs and IFN gamma also up-regulated SsMyD88 mRNA. Furthermore, ectopic expression of SsMyD88 in HEK cells was able to activate a NF-kappa B reporter construct indicating that the cloned salmon molecule was a functional MyD88 homologue. (C) 2009 Elsevier Ltd. All rights reserved.
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