期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 33, 期 2, 页码 176-186出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2008.08.006
关键词
Chinook salmon; Bacterial kidney disease; Cell-mediated immunity; Suppression subtractive hybridization
资金
- NWFSC
- NOAA Fisheries
An effective host response to Renibacterium salmoninarum, the etiologic agent of bacterial kidney disease, is poorly characterized. Using suppression subtractive hybridization, we exploited the difference in early host response in the pronephros of fish challenged by an attenuated strain (MT239) or a virulent strain (ATCC 33209) of R. salmoninarum. Among the 132 expressed sequence tag (EST) clones that were sequenced, 20 were selected for expression analysis at 24 and 72 h after challenge. ESTs matching two interferon inducible genes (IFN-inducible GBP and VLIG1), the ligand GAS6, and the kinase VRK2 were upregulated in fish exposed to MT239, but clownregulated or unchanged in fish exposed to 33209. A second group of ESTs matching genes involved in apoptosis (caspase 8) and immune function (I kappa B alpha, p47(phoX), EMR/CD97) were more slowly upregulated in fish exposed to 33209 compared to fish exposed to MT239. The ESTs displaying elevated expression in MT239-exposed fish may represent important cellular processes to bacterial challenge, and may be useful indicators of an effective host response to R. salmoninarum infection. Published by Elsevier Ltd.
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