期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 33, 期 10, 页码 1128-1136出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2009.06.003
关键词
Fish; Protozoa; Heat shock protein; hsp70; Parasite; Inflammation; Gene expression; Nitric oxide; Cytokines
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Department of Biological Sciences
We report on the cloning and characterization of Trypanosoma carassii 70 KDa heat shock protein (hsp70). T carassii hsp70 was secreted/excreted into culture medium in vitro and was recognized by sera from infected fish. Recombinant hsp70 (rhsp70) activated goldfish macrophages and stimulated the production of pro-inflammatory cytokines including interferon gamma (IFN gamma), tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, (IL)-12 and chemokines CCL-1 and CXCL-8 (IL-8). T carassii hsp70-induced cytokine expression was abrogated by pronase treatment of macrophages confirming the existence of receptor(s) on goldfish macrophage surface that recognize parasite molecule. Parasite hsp70 also up-regulated the expression inducible nitric oxide synthase (NOS) isoforms A and B and induced a strong nitric oxide response of goldfish macrophages. (C) 2009 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据