期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 32, 期 10, 页码 1221-1230出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2008.03.012
关键词
V(D)J recombination; SpRAG1L; SpRAG2L; RAG2; PHD domain; Strongylocentrotus purpuratus; H3K4 methylation; chromatin
资金
- Intramural NIH HHS [Z01 AG000388-01] Funding Source: Medline
- NATIONAL INSTITUTE ON AGING [Z01AG000388] Funding Source: NIH RePORTER
V(D)J recombination is a somatic gene rearrangement process that assembles antigen receptor genes from individual segments during lymphocyte development. The access of the RAG1/RAG2 recombinase to these gene segments is regulated at the level of chromatin modifications, in particular histone tail modifications. Trimethylation of lysine 4 in histone H3 (H3K4me3) correlates with actively recombining gene elements, and this mark is recognized and interpreted by the plant homeodomain (PHD) of RAG2. Here we report that the PHD domain of the only known invertebrate homolog of RAG2, the SpRAG2L protein of the purple sea urchin (Strongylocentrotus purpuratus) also binds to methylated histones, but with a unique preference for H3K4me2. While the cognate substrate for the sea urchin RAG1L/RAG2L complex remains elusive, the affinity for histone tails and the nuclear localization of ectopically expressed SpRAG2L strongly support the model that this enzyme complex exerts its activity on DNA in the context of chromatin. Published by Elsevier Ltd.
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