期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 32, 期 5, 页码 532-543出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2007.09.009
关键词
macrophages; tumor necrosis factor-alpha, TNF alpha; inflammation; fish; goldfish; cytokines; chemotaxis; phagocytosis; nitric oxide; respiratory burst
We identified and characterized two isoforms of tumor necrosis factor-alpha (TNF alpha) from the goldfish, TNFo alpha-1 and TNF alpha-2. At the protein level, goldfish TNF alpha-1 and TNF alpha-2 were most homologous to carp TNF alpha-1 and TNF alpha-2, respectively. Phytogenetically, the two goldfish isoforms grouped most closely with the carp TNF alpha soforms and TNF species of other cyprinids. Real-time PCR analysis revealed constitutive expression of goldfish TNF alpha-1 and TNF alpha-2 in all tissues with TNF alpha-2 mRNA levels higher than TNF alpha-1 in all tissues examined. A modest up-regutation in expressions of goldfish TNF alpha-1 and TNF alpha-2 in kidney-derived monocytes and significant increase in expression of both isoforms in mature macrophages were observed in response to activation with macrophage-activating factors. TNF alpha-2 was subsequently expressed using a prokaryotic expression system and the recombinant molecule (rTNF alpha-2) was functionally characterized. The rTNF alpha-2 induce a dose-dependent chemotactic response and enhanced phagocytosis of primary goldfish macrophages. Furthermore, rTNF alpha-2 primed the respiratory burst in monocytes and induced nitric oxide production of primary goldfish macrophages. Our results indicate that goldfish TNF alpha is a central regulatory and effector cytokine of inflammatory and antimicrobial responses of the goldfish. (c) 2007 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据