4.7 Article

p120-catenin-dependent junctional recruitment of Shroom3 is required for apical constriction during lens pit morphogenesis

期刊

DEVELOPMENT
卷 141, 期 16, 页码 3177-3187

出版社

COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.107433

关键词

Shroom3; Apical constriction; Invagination; Lens pit morphogenesis; p120-catenin; delta1 catenin (Ctnnd1)

资金

  1. National Institutes of Health [R01 EY016241, 5R01 GM102524, R01 GM067525]
  2. Abrahamson Pediatric Eye Institute of the Children's Hospital Medical Center of Cincinnati

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Apical constriction (AC) is a widely utilized mechanism of cell shape change whereby epithelial cells transform from a cylindrical to conical shape, which can facilitate morphogenetic movements during embryonic development. Invertebrate epithelial cells undergoing AC depend on the contraction of apical cortex-spanning actomyosin filaments that generate force on the apical junctions and pull them toward the middle of the cell, effectively reducing the apical circumference. A current challenge is to determine whether these mechanisms are conserved in vertebrates and to identify the molecules responsible for linking apical junctions with the AC machinery. Utilizing the developing mouse eye as a model, we have uncovered evidence that lens placode AC may be partially dependent on apically positioned myosin-containing filaments associated with the zonula adherens. In addition we found that, among several junctional components, p120-catenin genetically interacts with Shroom3, a protein required for AC during embryonic morphogenesis. Further analysis revealed that, similar to Shroom3, p120-catenin is required for AC of lens cells. Finally, we determined that p120-catenin functions by recruiting Shroom3 to adherens junctions. Together, these data identify a novel role for p120-catenin during AC and further define the mechanisms required for vertebrate AC.

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