期刊
DEVELOPMENT
卷 141, 期 23, 页码 4580-4589出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.111229
关键词
miR156; FLC; MED12; MED13; Mediator; Arabidopsis thaliana
资金
- US Department of Energy (DOE) grant [DE-FG02-99ER20328]
- National Institutes of Health (NIH) grant [RO1 GM51893]
- NIH NRSA postdoctoral fellowship [F32 GM069104]
- NIH NRSA [F32 GM075540]
- CONACyT PhD fellowship [326677]
- CINVESTAV institutional funds
- CONACyT [152333]
Temporal coordination of developmental programs is necessary for normal ontogeny, but the mechanism by which this is accomplished is still poorly understood. We have previously shown that two components of the Mediator CDK8 module encoded by CENTER CITY (CCT; Arabidopsis MED12) and GRAND CENTRAL (GCT; Arabidopsis MED13) are required for timing of pattern formation during embryogenesis. A morphological, molecular and genomic analysis of the post-embryonic phenotype of gct and cct mutants demonstrated that these genes also promote at least three subsequent developmental transitions: germination, vegetative phase change, and flowering. Genetic and molecular analyses indicate that GCT and CCT operate in parallel to gibberellic acid, a phytohormone known to regulate these same three transitions. We demonstrate that the delay in vegetative phase change in gct and cct is largely due to overexpression of miR156, and that the delay in flowering is due in part to upregulation of FLC. Thus, GCT and CCT coordinate vegetative and floral transitions by repressing the repressors miR156 and FLC. Our results suggest that MED12 and MED13 act as global regulators of developmental timing by fine-tuning the expression of temporal regulatory genes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据