期刊
DEVELOPMENT
卷 140, 期 6, 页码 1364-1368出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.091844
关键词
Clearing reagent; Whole mount; Retinal axon pathway; Immunohistochemistry; Fluorescent protein; Dil
资金
- National Institues of Health [R01 EY012736, EY015290, T32 EY013933, R01 EY012716]
- Uehara Foundation
We describe a clearing method for enhanced visualization of cell morphology and connections in neuronal and non-neuronal tissue. Using Clear(T) or Clear(T2), which are composed of formamide or formamide/polyethylene glycol, respectively, embryos, whole mounts and thick brain sections can be rapidly cleared with minimal volume changes. Unlike other available clearing techniques, these methods do not use detergents or solvents, and thus preserve lipophilic dyes, fluorescent tracers and immunohistochemical labeling, as well as fluorescent-protein labeling.
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