期刊
DEVELOPMENT
卷 140, 期 3, 页码 617-626出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.085969
关键词
Kif5b; Desmin; Nestin; Myofibrils; Myotendinous junctions
资金
- Hong Kong Research Grants Council [HKU 7321/04M, HKU 7636/05M, HKU 767110M]
- Hong Kong University Seed Funding Programme for Basic Research [200911159033]
- RGC Collaborative Research Fund [HKUST6/CRF/08]
Controlled delivery of myofibril components to the appropriate sites of assembly is crucial for myofibrillogenesis. Here, we show that kinesin-1 heavy chain Kif5b plays important roles in anterograde transport of alpha-sarcomeric actin, non-muscle myosin IIB, together with intermediate filament proteins desmin and nestin to the growing tips of the elongating myotubes. Mice with Kif5b conditionally knocked out in myogenic cells showed aggregation of actin filaments and intermediate filament proteins in the differentiating skeletal muscle cells, which further affected myofibril assembly and their linkage to the myotendinous junctions. The expression of Kif5b in mutant myotubes rescued the localization of the affected proteins. Functional mapping of Kif5b revealed a 64-amino acid alpha-helix domain in the tail region, which directly interacted with desmin and might be responsible for the transportation of these proteins in a complex.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据