期刊
DEVELOPMENT
卷 140, 期 3, 页码 667-674出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.088872
关键词
Cl.8; Cell culture media development; Growth; Imaginal; Proliferation; Wing disc
资金
- NCCR Frontiers-in-Genetics doctoral school
- EMBO Long-term fellowship
- Swiss National Science Foundation
- Kanton of Zurich
- SystemsX.ch initiative
- European Research Council under the European Community's Seventh Framework Programme
The Drosophila wing imaginal disc is a key model organ for molecular developmental genetics. Wing disc studies are generally restricted to end-point analyses of fixed tissues. Recently several studies have relied on limited data from discs cultured in uncharacterized conditions. Systematic efforts towards developing Drosophila organ culture techniques are becoming crucial for further progress. Here, we have designed a multi-tiered, high-throughput pipeline that employs design-of-experiment methods to design a culture medium for wing discs. The resulting formula sustains high levels of proliferation for more than 12 hours. This approach results in a statistical model of proliferation as a function of extrinsic growth supplements and identifies synergies that improve insulin-stimulated growth. A more dynamic view of organogenesis emerges from the optimized culture system that highlights important facets of growth: spatiotemporal clustering of cell divisions and cell junction rearrangements. The same approach could be used to improve culture conditions for other organ systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据