期刊
DEVELOPMENT
卷 139, 期 23, 页码 4484-4490出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.078790
关键词
Mouse; Embryo; IacZ; beta-Galactosidase; X-gal; S-gal; TNBT
资金
- National Institutes of Health [GM87130]
- Ben F. Love Endowment
- Kleberg Foundation
The bacterial IacZ gene is widely used as a reporter in a myriad of mouse transgenic experiments beta-Galactosidase, encoded by IacZ, is usually detected using X-gal in combination with ferric and ferrous ions. This assay produces a blue indole precipitate that is easy to detect visually. Here, we show that Salmon-gal in combination with tetrazolium salts provides a more sensitive and faster staining reaction than the traditional beta-galactosidase assay in mouse embryos. Using a combination of Salmon-gal and tetranitroblue tetrazolium, we were able to visualize the activity of beta-galactosidase in embryos at stages when the customary X-gal reaction failed to detect staining. Our studies provide an enhanced alternative for beta-galactosidase detection in expression and cell fate studies that use lacZ-based transgenic mouse lines.
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